International Journal on Magnetic Particle Imaging
Vol 8 No 1 Suppl 1 (2022): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2022.2203038

Proceedings Articles

Characterization of the Synomag®-D-PEG-OMe nanoparticles for the encapsulation in human and murine red blood cells

Main Article Content

Antonella Antonelli  (Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy), Emanuele Salvatore Scarpa  (Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy), Riccardo Di Corato  (Institute for Microelectronics and Microsystems (IMM), CNR, Via Monteroni, Lecce 73100, Italy), Florian Thieben  (Section for Biomedical Imaging, University Medical Center Hamburg-Eppendorf, Hamburg, Germany and Institute for Biomedical Imaging, Hamburg University of Technology, Hamburg, Germany), Cordula Grüttner (micromod Partikeltechnologie GmbH, Schillingallee 68, D-18057 Rostock, Germany), Tobias Knopp (Section for Biomedical Imaging, University Medical Center Hamburg-Eppendorf, Hamburg, Germany and Institute for Biomedical Imaging, Hamburg University of Technology, Hamburg, Germany), Mauro Magnani  (Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy)

Abstract

It was shown that the encapsulation of SPIO-based contrast agents in the red blood cells (RBCs) increases the circulation time in blood of these nanomaterials. Not all iron oxide particles are eligible for the entrapment into RBCs, depending on several factors and synthesis protocol. We have recently identified some type of nanoparticles that can be loaded with our method into RBCs to produce biocompatible SPIO-RBCs carriers that could be used as new intravascular tracers for biomedical applications, such as Magnetic Particle Imaging (MPI). Here, we report the first in vitro results obtained by using the Synomag®-D-PEG-OMe nanoparticles with both human and murine RBCs. MPS analysis showed that human Synomag®-D-PEG-OMe-loaded RBCs produced a signal that is weaker respect to the remarkable signal obtained with ferucarbotran loaded-RBCs prepared at the same condition, but it is to be noted that the encapsulation efficiency of Synomag®-D-PEG-OMe into cells is lower compared to ferucarbotran nanoparticles.

Article Details