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For a more detailed assessment of the performance of magnetic nanoparticles used as tracer in Magnetic Particle Imaging, we applied centrifugal flow-field fractionation (CF3) to separate a tracer according to their density and mass. The fractions were then magnetically and physically characterized by MPS, DLS, MALS and UV/Vis. Combining these findings with the results of magnetic characterization will allow a better understanding of the underlying mechanisms of MPI signal generation and tracer performance.