International Journal on Magnetic Particle Imaging
Vol 8 No 1 Suppl 1 (2022): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2022.2203070

Proceedings Articles

Rapid in situ labelling and tracking of neutrophils and macrophages to inflammation using antibody-functionalized MPI tracers

Main Article Content

K. L. Barry Fung  , Prashant Chandrasekharan , Xinyi Y. Zhou , Weiwen Cui , Lawrence Fong  , Steven Conolly 

Abstract

White blood cell (WBC) tracking is an imaging technique for clinical diagnoses of inflammation. WBC tracking utilises In-111 scintigraphy with ex vivo WBC labeling, which is cumbersome (~1 hr prep), requires hot chemistry, and kills most WBCs within 24 hours. Magnetic particle imaging (MPI) is a tracer imaging modality that detects superparamagnetic iron oxide nanoparticles (SPIOs), providing a sensitive (~200 cell), radiation-free alternative. As such, this work shows the first in situ labelling and tracking of WBCs to lipopolysaccharide-induced myositis using antibody-targeted MPI (Ab-MPI). SPIOs functionalized with Anti-Ly6G or Anti-F4/80 antibodies were used to track neutrophils or macrophages respectively, yielding differential dynamics and contrast (CNR = ~8-13) in imaging myositis vs. an untargeted control (ferucarbotran). These results showcase Ab-MPI as a radiation-free imaging platform for in situ targeting of immune cell speicific antigen epitopes.

Article Details

References

[1] I. Ak, E. Vardereli, B.Durak, Z.Gülba¸s, N. Basaran, M. P. Stokkel, and
E. K. Pauwels. Labeling of mixed leukocytes with 99mTc-HMPAO
causes severe chromosomal aberrations in lymphocytes. Journal
of NuclearMedicine, 43(2):203–206, 2002.
[2] B. Gleich and J.Weizenecker. Tomographic imaging using the nonlinear
response of magnetic particles.Nature, 435(7046):1214–1217,
2005, doi:10.1038/nature03808.
[3] P.W. Goodwill, E. U. Saritas, L. R. Croft, T. N. Kim, K. M. Krishnan,
D. V. Schaffer, and S. M. Conolly. X-Space MPI:Magnetic Nanoparticles
for SafeMedical Imaging. AdvancedMaterials, 24(28):3870–
3877, 2012, doi:10.1002/adma.201200221.
[4] E. Y. Yu, M. Bishop, B. Zheng, R. M. Ferguson, A. P. Khandhar,
S. J. Kemp, K. M. Krishnan, P. W. Goodwill, and S. M.
Conolly.Magnetic Particle Imaging: A Novel in Vivo Imaging Platform
for Cancer Detection. Nano Letters, 17(3):1648–1654, 2017,
doi:10.1021/acs.nanolett.6b04865.
[5] B. Zheng, M. P. von See, E. Yu, B. Gunel, K. Lu, T. Vazin, D. V. Schaffer,
P.W. Goodwill, and S. M. Conolly. QuantitativeMagnetic Particle
ImagingMonitors the Transplantation, Biodistribution, and
Clearance of Stem Cells In Vivo. Theranostics, 6(3):291–301, 2016,
doi:10.7150/thno.13728.
[6] F. Shen and J. K. Park. Toxicity Assessment of Iron Oxide Nanoparticles
Based on CellularMagnetic Loading UsingMagnetophoretic
Sorting in a Trapezoidal Microchannel. Analytical Chemistry,
90(1):920–927, 2018, doi:10.1021/acs.analchem.7b03875.
[7] J. C. Tseng and A. L. Kung. In vivo imaging method to distinguish
acute and chronic inflammation. Journal of visualized experiments
: JoVE, (78)pp. 1–5, 2013, doi:10.3791/50690.
[8] P. Chandrasekharan, K. B. Fung, X. Y. Zhou,W. Cui, C. Colson, . ..., L.
Fong, and S. M. Conolly. Non-radioactive and sensitive tracking of
neutrophils towards inflammation using antibody functionalized
magnetic particle imaging tracers. Nanotheranostics, 5(2):240–255,
2021, doi:10.7150/ntno.50721.
[9] C. Love, M. B. Tomas, G. G. Tronco, and C. J. Palestro. FDG PET
of Infection and Inflammation. RadioGraphics, 25(5):1357–1368,
2005, doi:10.1148/rg.255045122.

Most read articles by the same author(s)