International Journal on Magnetic Particle Imaging IJMPI
Vol. 9 No. 1 Suppl 1 (2023): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2023.2303063
Fast detection of Sars-CoV2 Antibodies by the use of critical off-set magnetic particle spectroscopy (COMPASS)
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Abstract
Current bioassays for detection of antibodies or antigens such as ELISA (Enzyme-linked Immunosorbent Assay) are relatively inflexible, expensive and time-consuming. Upcoming methods, such as ACS (AC susceptometry) or MPS (Magnetic Particle Spectroscopy), exploit the magnetization response of functionalized MNP ensembles to assess specific information about the MNP mobility as well as conjugations of chemical or biological compounds on their surface. Both methods have shown promising results in the past but cannot reach the sensitivity of above-mentioned techniques. We used a novel method based on a modified MPS being sensitive to minimal changes in mobility of MNP ensembles. This facilitates robust and easy-to-handle measurements of minimal changes in the diameter of MNPs. As an example, we detected SARS-CoV-2 antibodies binding to the S1 antigen on the surface of functionalized MNPs. Without any purification or incubation, we could show a sensitivity of less than 50 ng/mL of SARS-CoV-2 antibodies in human blood.
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References
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